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This article is part of a Special Issue entitled: Mass Spectrometry Imaging. Through this protocol, the amount of drug per gram of tissue was determined, which in turn, was compared with other analytical techniques such as Liquid Chromatography-Mass spectrometry (LC-MS/MS). This factor takes into account the ion suppression effect that is both tissue- and drug-specific. MS also produces altered responsiveness to and self-administration (SA) of various drugs of abuse including cocaine, ethanol, and amphetamine. Animals subjected to MS during the first 2 weeks of life display altered behavioral and neuroendocrinological stress responses as adults. To address these challenges, we based our approach on the use of a normalization factor called the TEC (Tissue Extinction Coefficient). The maternal separation (MS) paradigm is an animal model of early life stress. These include the high dependence of the detected signal on the matrix deposition, the MALDI ionization yield of specific target molecules, and lastly, the ion suppression effect on the tissue. We describe a method intended to respond to the main challenges encountered in quantification through MALDI imaging dedicated to whole-body or single heterogeneous organ samples (brain, eye, liver). In order to quantify small molecules at the early stage of drug discovery, we developed a quantitation approach based on mass spectrometry imaging (MSI) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) without the use of a labeled compound. Stauber,a)Ī) ImaBiotech, MS Imaging Department, Lille, France

“,”Hamm G, Bonnel D, Legouffe R, Pamelard F, Delbos J, Bouzom F, Stauber J”,”(G. Quantitative mass spectrometry imaging of propranolol and olanzapine using tissue extinction calculation as normalization factor

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